The Saccharomyces cerevisiae CDC25 gene encodes a guanine nucleotide exchange factor for Ras proteins whose catalytic domain is highly homologous to Ras-guanine nucleotide exchange factors from higher eukaryotes. In this study, glucose-induced Ras activation and cAMP response were investigated in mutants lacking the N-terminal domain of Cdc25 or where the entire CDC25 coding sequence was substituted by an expression cassette for a mammalian guanine nucleotide exchange factor catalytic domain. Our results suggest that an unregulated, low Ras guanine nucleotide exchange factor activity allows a normal glucose-induced cAMP signal that appears to be mediated mainly by the Gpr1/Gpa2 system, but it was not enough to sustain the glucose-induced increase of Ras2-GTP normally observed in a wild-type strain.

Paiardi, C., Belotti, F., Colombo, S., Tisi, R., Martegani, E. (2007). The large N-terminal domain of Cdc25 protein of the yeast S. cerevisiae is required for glucose-induced Ras2 activation. FEMS YEAST RESEARCH, 7(8), 1270-1275 [10.1111/j.1567-1364.2007.00300.x].

The large N-terminal domain of Cdc25 protein of the yeast S. cerevisiae is required for glucose-induced Ras2 activation.

COLOMBO, SONIA;TISI, RENATA ANITA;MARTEGANI, ENZO
2007

Abstract

The Saccharomyces cerevisiae CDC25 gene encodes a guanine nucleotide exchange factor for Ras proteins whose catalytic domain is highly homologous to Ras-guanine nucleotide exchange factors from higher eukaryotes. In this study, glucose-induced Ras activation and cAMP response were investigated in mutants lacking the N-terminal domain of Cdc25 or where the entire CDC25 coding sequence was substituted by an expression cassette for a mammalian guanine nucleotide exchange factor catalytic domain. Our results suggest that an unregulated, low Ras guanine nucleotide exchange factor activity allows a normal glucose-induced cAMP signal that appears to be mediated mainly by the Gpr1/Gpa2 system, but it was not enough to sustain the glucose-induced increase of Ras2-GTP normally observed in a wild-type strain.
Articolo in rivista - Articolo scientifico
Cdc25; RasGTP; cAMP; Saccharomyces cerevisiae
English
29-ago-2007
7
8
1270
1275
none
Paiardi, C., Belotti, F., Colombo, S., Tisi, R., Martegani, E. (2007). The large N-terminal domain of Cdc25 protein of the yeast S. cerevisiae is required for glucose-induced Ras2 activation. FEMS YEAST RESEARCH, 7(8), 1270-1275 [10.1111/j.1567-1364.2007.00300.x].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/1616
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