Here, we describe the use of complementary techniques applicable to different types of samples to analyze chromosomal alterations in urothelial carcinoma. By a conventional chromosome analysis on fresh biopsies, it is possible to delineate the status of ploidy and rough chromosomal aberrations. The multi-target fluorescence in situ hybridization (FISH) UroVysion test, for the rapid detection of chromosomal aneusomy of chromosomes 3, 7, and 17 and/or deletion of 9p21 locus, is applicable to urine specimens as well as to formalin-fixed paraffin-embedded (FFPE) specimens and fresh biopsies. Finally, array comparative genomic hybridization (array-CGH) gives the possibility of analyzing the DNA in a single experiment from a biopsy of the tumor but also from FFPE specimens; this technique is able to detect alterations at the genome level not excluding any chromosome.

Conconi, D., Bentivegna, A. (2018). Analysis of chromosomal alterations in urothelial carcinoma. In Methods in Molecular Biology: Urothelial Carcinoma (pp. 3-17). Humana Press Inc. [10.1007/978-1-4939-7234-0_1].

Analysis of chromosomal alterations in urothelial carcinoma

Conconi, D
Primo
;
Bentivegna, A
Ultimo
2018

Abstract

Here, we describe the use of complementary techniques applicable to different types of samples to analyze chromosomal alterations in urothelial carcinoma. By a conventional chromosome analysis on fresh biopsies, it is possible to delineate the status of ploidy and rough chromosomal aberrations. The multi-target fluorescence in situ hybridization (FISH) UroVysion test, for the rapid detection of chromosomal aneusomy of chromosomes 3, 7, and 17 and/or deletion of 9p21 locus, is applicable to urine specimens as well as to formalin-fixed paraffin-embedded (FFPE) specimens and fresh biopsies. Finally, array comparative genomic hybridization (array-CGH) gives the possibility of analyzing the DNA in a single experiment from a biopsy of the tumor but also from FFPE specimens; this technique is able to detect alterations at the genome level not excluding any chromosome.
Capitolo o saggio
Chromosome aberrations; Comparative Genomic Hybridization (array-CGH); DNA copy number variations; Fluorescence in situ hybridization (FISH); Formalin-fixed paraffin-embedded (FFPE) specimens; Fresh biopsies; Urine specimens; Urothelial carcinoma; UroVysion test;
urothelial carcinoma, chromosome aberrations, fluorescence in situ hybridization (FISH), UroVysion test, Comparative Genomic Hybridization (array-CGH), DNA Copy Number Variations, urine specimens, fresh biopsies, formalin fixed paraffin embedded (FFPE) specimens
English
Methods in Molecular Biology: Urothelial Carcinoma
2018
978-1-4939-7234-0
1655
Humana Press Inc.
3
17
Conconi, D., Bentivegna, A. (2018). Analysis of chromosomal alterations in urothelial carcinoma. In Methods in Molecular Biology: Urothelial Carcinoma (pp. 3-17). Humana Press Inc. [10.1007/978-1-4939-7234-0_1].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/154801
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