Skeletal muscle regeneration is a physiological process that occurs in adult skeletal muscles in response to injury or disease. Acute injury-induced skeletal muscle regeneration is a widely used, powerful model system to study the events involved in muscle regeneration as well as the mechanisms and different players. Indeed, a detailed knowledge of this process is essential for a better understanding of the pathological conditions that lead to skeletal muscle degeneration, and it aids in identifying new targeted therapeutic strategies. The present work describes a detailed and reproducible protocol to induce acute skeletal muscle regeneration in mice through a single intramuscular injection of cardiotoxin (CTX). CTX belongs to the family of snake venom toxins and causes myolysis of myofibers, which eventually triggers the regeneration events. The dynamics of skeletal muscle regeneration is evaluated by histological analysis of muscle sections. The protocol also illustrates the experimental procedures for dissecting, freezing, and cutting the Tibialis Anterior muscle, as well as the routine Hematoxylin & Eosin staining that is widely used for subsequent morphological and morphometric analysis.

Guardiola, O., Andolfi, G., Tirone, M., Iavarone, F., Brunelli, S., Minchiotti, G. (2017). Induction of acute skeletal muscle regeneration by cardiotoxin injection. JOURNAL OF VISUALIZED EXPERIMENTS, 2017(119), 1-6 [10.3791/54515].

Induction of acute skeletal muscle regeneration by cardiotoxin injection

Tirone, M;Brunelli, S;
2017

Abstract

Skeletal muscle regeneration is a physiological process that occurs in adult skeletal muscles in response to injury or disease. Acute injury-induced skeletal muscle regeneration is a widely used, powerful model system to study the events involved in muscle regeneration as well as the mechanisms and different players. Indeed, a detailed knowledge of this process is essential for a better understanding of the pathological conditions that lead to skeletal muscle degeneration, and it aids in identifying new targeted therapeutic strategies. The present work describes a detailed and reproducible protocol to induce acute skeletal muscle regeneration in mice through a single intramuscular injection of cardiotoxin (CTX). CTX belongs to the family of snake venom toxins and causes myolysis of myofibers, which eventually triggers the regeneration events. The dynamics of skeletal muscle regeneration is evaluated by histological analysis of muscle sections. The protocol also illustrates the experimental procedures for dissecting, freezing, and cutting the Tibialis Anterior muscle, as well as the routine Hematoxylin & Eosin staining that is widely used for subsequent morphological and morphometric analysis.
Articolo in rivista - Articolo scientifico
Acute skeletal muscle regeneration; Cardiotoxin; Developmental biology; Fresh frozen muscle technique; Hematoxylin & eosin staining; Issue 119; Myofibers cross sectional area; Tibialis anterior muscle isolation;
muscle regeneration
English
2017
2017
119
1
6
e54515
reserved
Guardiola, O., Andolfi, G., Tirone, M., Iavarone, F., Brunelli, S., Minchiotti, G. (2017). Induction of acute skeletal muscle regeneration by cardiotoxin injection. JOURNAL OF VISUALIZED EXPERIMENTS, 2017(119), 1-6 [10.3791/54515].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/142222
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