A new photoactivable, radioactive derivative of ganglioside GM1 has been utilized to assess lipid distribution in the caveolae bilayer, taking advantage of the ability of the glycolipid, endogenous or exogenously added, to concentrate within this membrane compartment and to crosslink neighboring molecules upon illumination. After insertion into A431 plasma membrane and photoactivation, a membrane-enriched and a detergent-resistant fraction, enriched in gangliosides, sphingomyelin and cholesterol, were isolated. While a few radioactive proteins were detected in the membrane-enriched fraction, only radioactive caveolin was detected in the detergent-resistant fraction, indicating at the same time the enrichment of this fraction In caveolae and the presence of ganglioside within this compartment Among lipids, crosslinked phosphatidylcholine, sphingomyelin and cholesterol were detected in the membrane-enriched fraction, while only crosslinked sphingomyelin was detected in the detergent-resistant fraction. These results suggest the enrichment in sphingomyelin-along with ganglioside-within the outer leaflet, and the preferential localization of cholesterol within the endoplasmic leaflet, of the caveolae bilayer

Pitto, M., Brunner, J., Ferraretto, A., Ravasi, D., Palestini, P., Masserini, M. (2000). Use of a photoactivable GM1 ganglioside analogue to assess lipid distribution in caveolae bilayer. GLYCOCONJUGATE JOURNAL, 17(3-4), 215-222 [10.1023/A:1026593307882].

Use of a photoactivable GM1 ganglioside analogue to assess lipid distribution in caveolae bilayer

PITTO, MARINA;PALESTINI, PAOLA NOVERINA ADA;MASSERINI, MASSIMO ERNESTO
2000

Abstract

A new photoactivable, radioactive derivative of ganglioside GM1 has been utilized to assess lipid distribution in the caveolae bilayer, taking advantage of the ability of the glycolipid, endogenous or exogenously added, to concentrate within this membrane compartment and to crosslink neighboring molecules upon illumination. After insertion into A431 plasma membrane and photoactivation, a membrane-enriched and a detergent-resistant fraction, enriched in gangliosides, sphingomyelin and cholesterol, were isolated. While a few radioactive proteins were detected in the membrane-enriched fraction, only radioactive caveolin was detected in the detergent-resistant fraction, indicating at the same time the enrichment of this fraction In caveolae and the presence of ganglioside within this compartment Among lipids, crosslinked phosphatidylcholine, sphingomyelin and cholesterol were detected in the membrane-enriched fraction, while only crosslinked sphingomyelin was detected in the detergent-resistant fraction. These results suggest the enrichment in sphingomyelin-along with ganglioside-within the outer leaflet, and the preferential localization of cholesterol within the endoplasmic leaflet, of the caveolae bilayer
Articolo in rivista - Articolo scientifico
Caveolae; GM1 ganglioside; Plasma membrane
English
2000
17
3-4
215
222
none
Pitto, M., Brunner, J., Ferraretto, A., Ravasi, D., Palestini, P., Masserini, M. (2000). Use of a photoactivable GM1 ganglioside analogue to assess lipid distribution in caveolae bilayer. GLYCOCONJUGATE JOURNAL, 17(3-4), 215-222 [10.1023/A:1026593307882].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/1421
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