Objectives: To evaluate the prevalence and clinical significance of cathepsin G antibodies in patients affected with systemic sclerosis (SSc, scleroderma). Methods: 115 patients affected by SSc, 55 (47,8%) with diffuse scleroderma (dSSc) and 60 (52,2%) with limited scleroderma (ISSc), were tested for cathepsin G antibodies by ELISA method. Moreover these sera were evaluated by indirect immunofluorescence (IIF) on ethanol and formalin fixed human neutrophils. Results: By means of the ELISA method 16 (13,9%) patients were found to be sera positive for anti-cathepsin G, 2 (12.5%) of which showed a perinuclear fluorescence pattern (P-ANCA) and 4 (25%) an atypical ANCA staining, while 10 (62,5%) were negative on IIF. The IIF on scleroderma sera revealed 5 (4,3%) P-ANCA and 18 (15,7%) atypical ANCA patterns. The anti-cathepsin G antibodies significantly prevailed in scleroderma sera (p=0.02) when their frequency was compared with that of healthy controls; while they were not significantly associated to any clinical or serological features of SSc patients. Conclusions: The anti-cathepsin G antibodies were significantly, frequent in scleroderma sera; however, no clinical correlations were found. Thus, the significance of their presence in SSc still needs to be clarified

Grypiotis, P., Ruffatti, A., Cozzi, F., Sinico, R., Tonello, M., Radice, A., et al. (2003). [Prevalence and clinical significance of cathepsin G antibodies in systemic sclerosis]. REUMATISMO, 55(4), 256-262.

[Prevalence and clinical significance of cathepsin G antibodies in systemic sclerosis]

SINICO, RENATO ALBERTO;
2003

Abstract

Objectives: To evaluate the prevalence and clinical significance of cathepsin G antibodies in patients affected with systemic sclerosis (SSc, scleroderma). Methods: 115 patients affected by SSc, 55 (47,8%) with diffuse scleroderma (dSSc) and 60 (52,2%) with limited scleroderma (ISSc), were tested for cathepsin G antibodies by ELISA method. Moreover these sera were evaluated by indirect immunofluorescence (IIF) on ethanol and formalin fixed human neutrophils. Results: By means of the ELISA method 16 (13,9%) patients were found to be sera positive for anti-cathepsin G, 2 (12.5%) of which showed a perinuclear fluorescence pattern (P-ANCA) and 4 (25%) an atypical ANCA staining, while 10 (62,5%) were negative on IIF. The IIF on scleroderma sera revealed 5 (4,3%) P-ANCA and 18 (15,7%) atypical ANCA patterns. The anti-cathepsin G antibodies significantly prevailed in scleroderma sera (p=0.02) when their frequency was compared with that of healthy controls; while they were not significantly associated to any clinical or serological features of SSc patients. Conclusions: The anti-cathepsin G antibodies were significantly, frequent in scleroderma sera; however, no clinical correlations were found. Thus, the significance of their presence in SSc still needs to be clarified
Articolo in rivista - Articolo scientifico
Adult; Aged; Autoantibodies; Cathepsin G; Cathepsins; Female; Humans; Male; Middle Aged; Prevalence; Scleroderma, Systemic; Serine Endopeptidases
Italian
2003
55
4
256
262
none
Grypiotis, P., Ruffatti, A., Cozzi, F., Sinico, R., Tonello, M., Radice, A., et al. (2003). [Prevalence and clinical significance of cathepsin G antibodies in systemic sclerosis]. REUMATISMO, 55(4), 256-262.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/139418
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