CK2 is a highly conserved protein kinase controlling different cellular processes. It shows a higher activity in proliferating mammalian cells, in various types of cancer cell lines and tumors. The findings presented herein provide the first evidence of an in vivo modulation of CK2 activity, dependent on growth rate, in Saccharomyces cerevisiae. In fact, CK2 activity, assayed on nuclear extracts, is shown to increase in expo- nential growing batch cultures at faster growth rate, while localization of catalytic and regulatory sub- units is not nutritionally modulated. Differences in intracellular CK2 activity of glucose- and ethanol- grown cells appear to depend on both increase in molecule number and kcat. Also in chemostat cultures nuclear CK2 activity is higher in faster growing cells providing the first unequivocal demonstration that growth rate itself can affect CK2 activity in a eukaryotic organism

Tripodi, F., Cirulli, C., Reghellin, V., Marin, O., Brambilla, L., Schiappelli, M., et al. (2010). CK2 activity is modulated by growth rate in Saccharomyces cerevisiae. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 398(1), 44-50 [10.1016/j.bbrc.2010.06.028].

CK2 activity is modulated by growth rate in Saccharomyces cerevisiae

TRIPODI, FARIDA;REGHELLIN, VERONICA;BRAMBILLA, LUCA GIUSEPPE;PORRO, DANILO;VANONI, MARCO ERCOLE;ALBERGHINA, LILIA;COCCETTI, PAOLA
2010

Abstract

CK2 is a highly conserved protein kinase controlling different cellular processes. It shows a higher activity in proliferating mammalian cells, in various types of cancer cell lines and tumors. The findings presented herein provide the first evidence of an in vivo modulation of CK2 activity, dependent on growth rate, in Saccharomyces cerevisiae. In fact, CK2 activity, assayed on nuclear extracts, is shown to increase in expo- nential growing batch cultures at faster growth rate, while localization of catalytic and regulatory sub- units is not nutritionally modulated. Differences in intracellular CK2 activity of glucose- and ethanol- grown cells appear to depend on both increase in molecule number and kcat. Also in chemostat cultures nuclear CK2 activity is higher in faster growing cells providing the first unequivocal demonstration that growth rate itself can affect CK2 activity in a eukaryotic organism
Articolo in rivista - Articolo scientifico
Saccharomyces cerevisiae, Growth rate, Carbon source, Phosphorylation
English
2010
398
1
44
50
none
Tripodi, F., Cirulli, C., Reghellin, V., Marin, O., Brambilla, L., Schiappelli, M., et al. (2010). CK2 activity is modulated by growth rate in Saccharomyces cerevisiae. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 398(1), 44-50 [10.1016/j.bbrc.2010.06.028].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/12408
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