We report the results of a microspectroscopy study on the Fourier transform infrared (FT-IR) absorption spectra of Caenorhabditis elegans, collected from the different parts of a single intact specimen - pharynx, intestine and tail regions. The principal absorption bands were assigned to the molecular species present in C. elegans, with an excellent reproducibility for the pharynx spectrum. These results enabled us to explore if FT-IR microspectroscopy could offer a new tool for nematode identification. As an example, the discrimination among four well characterised nematode taxa is reported. The FT-IR results completely match those obtained by Blaxter and colleagues through molecular biology [Nature 392 (1998) 71]. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

Ami, D., Natalello, A., Zullini, A., Doglia, S. (2004). Fourier transform infrared microspectroscopy as a new tool for nematode studies. FEBS LETTERS, 576(3), 297-300 [10.1016/j.febslet.2004.09.022].

Fourier transform infrared microspectroscopy as a new tool for nematode studies

AMI, DILETTA;NATALELLO, ANTONINO;ZULLINI, ALDO;DOGLIA, SILVIA MARIA
2004

Abstract

We report the results of a microspectroscopy study on the Fourier transform infrared (FT-IR) absorption spectra of Caenorhabditis elegans, collected from the different parts of a single intact specimen - pharynx, intestine and tail regions. The principal absorption bands were assigned to the molecular species present in C. elegans, with an excellent reproducibility for the pharynx spectrum. These results enabled us to explore if FT-IR microspectroscopy could offer a new tool for nematode identification. As an example, the discrimination among four well characterised nematode taxa is reported. The FT-IR results completely match those obtained by Blaxter and colleagues through molecular biology [Nature 392 (1998) 71]. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
Articolo in rivista - Articolo scientifico
Fourier transform infrared micro spectroscopy as a new tool for nematode studies
English
ott-2004
576
3
297
300
none
Ami, D., Natalello, A., Zullini, A., Doglia, S. (2004). Fourier transform infrared microspectroscopy as a new tool for nematode studies. FEBS LETTERS, 576(3), 297-300 [10.1016/j.febslet.2004.09.022].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/1081
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