Two recombinant Escherichia coli strains expressing different levels of an interferon fusion protein as inclusion bodies have been studied by Fourier transform infrared (FT-IR) microspectroscopy. A marker band at 1628 cm(-1) allowed monitoring of the protein expression by direct analysis of cell pellets in a rapid, non-invasive and quantitative way. The results demonstrate that FT-IR microspectroscopy is a technique of potential biotechnological. interest for studying inclusion body formation. (C) 2003 Elsevier B.V. All rights reserved

Ami, D., Bonecchi, L., Cali, S., Orsini, G., Tonon, G., Doglia, S. (2003). FT-IR study of heterologous protein expression in recombinant Escherichia coli strains. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1624(1-3), 6-10 [10.1016/j.bbagen.2003.09.008].

FT-IR study of heterologous protein expression in recombinant Escherichia coli strains

Ami, D;Doglia, SM
2003

Abstract

Two recombinant Escherichia coli strains expressing different levels of an interferon fusion protein as inclusion bodies have been studied by Fourier transform infrared (FT-IR) microspectroscopy. A marker band at 1628 cm(-1) allowed monitoring of the protein expression by direct analysis of cell pellets in a rapid, non-invasive and quantitative way. The results demonstrate that FT-IR microspectroscopy is a technique of potential biotechnological. interest for studying inclusion body formation. (C) 2003 Elsevier B.V. All rights reserved
Articolo in rivista - Articolo scientifico
inclusion body; FT-IR microspectroscopy; Escherichia coli; expression of heterologous protein; interferon alpha
English
2003
1624
1-3
6
10
none
Ami, D., Bonecchi, L., Cali, S., Orsini, G., Tonon, G., Doglia, S. (2003). FT-IR study of heterologous protein expression in recombinant Escherichia coli strains. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1624(1-3), 6-10 [10.1016/j.bbagen.2003.09.008].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/1048
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